產(chǎn)品說明 ：
產(chǎn)品貨號Reference: CAN-FT4-6040
檢測方法Kit Type: Competitive LIA
規(guī)格Kit Size: 96-well break-apart microplate
靈敏度Sensitivity: 1 pg/mL
檢測樣本Sample Type: Human serum / 25 μL
校準(zhǔn)范圍Calibrator Range: 2–80 pg/mL
檢測時間Total Assay Time: 90 minutes
背景介紹:
甲狀腺素(T4)是主要的甲狀腺激素，在血液中幾乎完全與載體蛋白結(jié)合。然而，只有游離的(未結(jié)合的)甲狀腺素被認(rèn)為是生物活性的。甲狀腺素的主要攜帶者是甲狀腺素結(jié)合球蛋白(TBG)、前白蛋白和白蛋白。與總甲狀腺素水平相比，游離甲狀腺素(FT4)水平與臨床狀態(tài)的相關(guān)性更好。與平衡透析法和超滲濾法相比，DbC游離法T4測定是一種快速、簡便的一步競爭液相免疫分析方法，且操作繁瑣、耗時，是一種快速、簡便的一步競爭LIA分析方法。該系統(tǒng)使用了高特異性的單克隆抗體和一種非模擬示蹤劑，實驗證明該示蹤劑沒有?不能與TBG和白蛋白結(jié)合的信號。正常甲狀腺組織中游離T4濃度為7~22pg/mL。甲狀腺功能減退癥患者游離T4水平降低，而甲狀腺功能亢進(jìn)癥患者游離T4水平升高。該方法有時與其他甲狀腺試驗一起用于體外診斷和評估接受甲狀腺治療的患者。
Thyroxine (T4), the principal thyroid hormone, circulates in blood almost completely bound to carrier proteins. However, only the free (unbound) fraction of thyroxine is considered to be biologically active. The main carriers of thyroxine are thyroxine-binding globulin (TBG), pre-albumin and albumin. The measurement of free thyroxine (fT4) levels correlate better with the clinical status than total thyroxine levels.
The DBC free T4 assay is a one step competitive LIA system that is rapid and easy to perform compared to equilibrium dialysis and ultra? ltration methods, which are cumbersome and time-consuming. This system employs a highly speci? c monoclonal antibody and a non-analog tracer that was proved experimentally to have no signi? cant binding to TBG and albumin.
In the euthyroid, normal population the free T4 concentration is 7–22 pg/mL. The level of free T4 is decreased in hypothyroidism while in thyrotoxic patients the level of free T4 is increased.
This assay is used at times with other thyroid tests for in vitro diagnostic purposes and for assessing patients who are receiving thyroid treatments (follow-up).
注：本產(chǎn)品僅用于科研用途，不能直接用于人體或臨床診斷使用。
