華拓生物公司是一家專業(yè)從事于抗體抗原、多肽等產(chǎn)品的研發(fā)生產(chǎn)、定制合成和銷售的專業(yè)高科技企業(yè)。公司致力于尖端分子生物學(xué)、免疫學(xué)、蛋白質(zhì)組學(xué)、細(xì)胞生物學(xué)技術(shù)在科研中的應(yīng)用與推廣,研制方向涉及抗體抗原、多肽產(chǎn)品、藥用輔料、綠色化學(xué)、手性化學(xué)等領(lǐng)域,面向社會(huì)開展產(chǎn)品研制和科技創(chuàng)新活動(dòng),為廣大企業(yè)提供高科技產(chǎn)品,其中抗體抗原、多肽、新型環(huán)保的殺菌劑已開始規(guī)模生產(chǎn)。
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服務(wù)類別 Package options |
服務(wù)內(nèi)容 Service Items |
服務(wù)內(nèi)容 Service Items |
交付產(chǎn)品 Delivery |
周期 Time |
多抗1(從蛋白抗原到抗血清) (From protein to antisera) |
檢測(cè)蛋白的純度和濃度 動(dòng)物免疫及血清采集 ELISA檢測(cè) |
protein checked by SDS-PAGE immunization and sera collection ELISA identification |
40-60ml抗血清 1-2ml免疫前血清 ELISA檢測(cè)結(jié)果 完整項(xiàng)目報(bào)告 |
10-12周 |
多抗2(從蛋白抗原到抗原親和純化) (From protein to antigen purified antibodies) |
檢測(cè)蛋白的純度和濃度 動(dòng)物免疫及血清采集 ELISA檢測(cè) 抗原親和純化 |
protein checked by SDS-PAGE immunization and sera collection ELISA identification antigen affinity purification |
2-5mg抗原親和純化抗體 1-2ml免疫前血清 ELISA檢測(cè)結(jié)果 完整項(xiàng)目報(bào)告 |
10-12周 |
多抗 3(從多肽設(shè)計(jì)到抗血清) (From peptide design to antisera) |
多肽設(shè)計(jì)與合成(12-22aa,85%) 多肽偶聯(lián) 動(dòng)物免疫及血清采集 ELISA檢測(cè) |
peptide design and synthesis(up to 22aa,85%) peptide conjugation immunization and sera collection ELISA identification |
2-3mg多肽 40-60ml抗血清 1-2ml免疫前血清 ELISA檢測(cè)結(jié)果 完整項(xiàng)目報(bào)告 |
12-15周 |
多抗 4(從多肽設(shè)計(jì)到抗原親和純化) (From peptide design to antigen purified antibodies) |
多肽設(shè)計(jì)與合成(12-22aa,85%) 多肽偶聯(lián) 動(dòng)物免疫及血清采集 ELISA檢測(cè) 抗原親和純化 |
peptide design and synthesis(up to 22aa,85%) peptide conjugation immunization and sera collection ELISA identification antigen affinity purification |
2-3mg多肽 2-5mg抗原親和純化抗體 1-2ml免疫前血清 ELISA檢測(cè)結(jié)果 完整項(xiàng)目報(bào)告 |
12-15周 |
多抗5(從質(zhì)粒到抗血清) (From plasmid to antisera) |
蛋白表達(dá)與純化 動(dòng)物免疫及血清采集 ELISA檢測(cè) |
protein expression and purification immunization and sera collection ELISA identification |
1mg表達(dá)蛋白 40-60ml抗血清 1-2ml免疫前血清 ELISA檢測(cè)結(jié)果 完整項(xiàng)目報(bào)告 |
12-15周 |
多抗6(從質(zhì)粒到抗原親和純化) (From plasmid to antigen purified antibodies) |
蛋白表達(dá)與純化 動(dòng)物免疫及血清采集 ELISA檢測(cè) 抗原親和純化 |
protein expression and purification immunization and sera collection ELISA identification antigen affinity purification |
1mg表達(dá)蛋白 2-5mg抗原親和純化抗體1-2ml免疫前血清 ELISA檢測(cè)結(jié)果 完整項(xiàng)目報(bào)告 |
12-15周 |
多抗 7(從cDNA到抗血清) (From cDNA to antisera) |
基因擴(kuò)增 載體構(gòu)建 蛋白表達(dá)與純化 動(dòng)物免疫及血清采集 ELISA檢測(cè) |
gene amplification vector construction protein expression and purification immunization and sera collection ELISA identification |
構(gòu)建的表達(dá)載體 1mg表達(dá)蛋白 40-60ml抗血清 1-2ml免疫前血清 ELISA檢測(cè)結(jié)果 完整項(xiàng)目報(bào)告 |
14-16周 |
多抗 8(從cDNA到抗原親和純化) (From cDNA to antigen purified antibodies) |
基因擴(kuò)增 載體構(gòu)建 蛋白表達(dá)與純化 動(dòng)物免疫及血清采集 ELISA檢測(cè) 抗原親和純化 |
gene amplification vector construction protein expression and purification immunization and sera collection ELISA identification antigen affinity purification |
構(gòu)建的表達(dá)載體 1mg表達(dá)蛋白 2-5mg抗原親和純化抗體 1-2ml免疫前血清 ELISA檢測(cè)結(jié)果 完整項(xiàng)目報(bào)告 |
14-16周 |
多抗 9(磷酸化多抗制備) (Phospho- specific polyclonal antibodies) |
多肽設(shè)計(jì)與合成(12-22aa,85%) 多肽偶聯(lián) 動(dòng)物免疫及血清采集 ELISA檢測(cè) 抗原親和純化 |
peptide design and synthesis(up to 22aa,85%) peptide conjugation immunization and sera collection ELISA identification antigen affinity purification |
2-3mg多肽(非磷酸化和磷酸化) 2-5mg磷酸化抗體 1-2ml免疫前血清 ELISA檢測(cè)結(jié)果 完整項(xiàng)目報(bào)告 |
13-16周 |
多抗 10 (Lys甲基化多抗制備) (Lys trimethylated specific polyclonal antibody) |
多肽設(shè)計(jì)與合成(12-22aa,85%) 多肽偶聯(lián) 動(dòng)物免疫及血清采集 ELISA檢測(cè) 抗原親和純化 |
Customer provides antigen peptide information (or we provide candidate sequences of peptide antigens for customer's approval.) Synthesize one peptide (up to 22aa,75%); Conjugate the peptide to carrier protein (KLH, BSA or OVA); Immunize two New Zealand rabbits; Titer the immunization by ELISA; Post-bleed collection. |
2-3mg多肽(非甲基化和甲基化) 2-5mg甲基化抗體 1-2ml免疫前血清 ELISA檢測(cè)結(jié)果 完整項(xiàng)目報(bào)告 |
13-16周 |
多抗 11 (Arg甲基化多抗制備) (Arg methylation-specific polyclonal antibody) |
多肽設(shè)計(jì)與合成(12-22aa,85%) 多肽偶聯(lián) 動(dòng)物免疫及血清采集 ELISA檢測(cè) 抗原親和純化 |
Customer provides antigen peptide information (or we provide candidate sequences of peptide antigens for customer's approval.) Synthesize one peptide (up to 22aa,75%); Conjugate the peptide to carrier protein (KLH, BSA or OVA); Immunize two New Zealand rabbits; Titer the immunization by ELISA; Post-bleed collection; Antigen affinity purification. |
2-3mg多肽(非甲基化和甲基化) 2-5mg甲基化抗體 1-2ml免疫前血清 ELISA檢測(cè)結(jié)果 完整項(xiàng)目報(bào)告 |
13-16周 |
多抗 12(Arg雙甲基化(對(duì)稱)多抗制備) (Arg di-methylation (Symmetry)-specific polyclonal antibody) |
多肽設(shè)計(jì)與合成(12-22aa,75%) 多肽偶聯(lián) 動(dòng)物免疫及血清采集 ELISA檢測(cè) 抗原親和純化 |
Customer provides antigen peptide information (or we provide candidate sequences of peptide antigens for customer's approval.) Synthesize one peptide (up to 22aa,75%); Conjugate the peptide to carrier protein (KLH, BSA or OVA); Immunize two New Zealand rabbits; Titer the immunization by ELISA; Post-bleed collection; Antigen affinity purification. |
2-3mg多肽(非甲基化和甲基化) 2-5mg甲基化抗體 1-2ml免疫前血清 ELISA檢測(cè)結(jié)果 完整項(xiàng)目報(bào)告 |
13-16周 |
多抗13 (Arg di-methylation (Asymmetric)-specific polyclonal antibody) |
多肽設(shè)計(jì)與合成(12-22aa,75%) 多肽偶聯(lián) 動(dòng)物免疫及血清采集 ELISA檢測(cè) 抗原親和純化 |
Customer provides antigen peptide information (or we provide candidate sequences of peptide antigens for customer's approval.) Synthesize one peptide (up to 22aa,75%); Conjugate the peptide to carrier protein (KLH, BSA or OVA); Immunize two New Zealand rabbits; Titer the immunization by ELISA; Post-bleed collection; Antigen affinity purification. |
2-3mg多肽(非甲基化和甲基化) 2-5mg甲基化抗體 1-2ml免疫前血清 ELISA檢測(cè)結(jié)果 完整項(xiàng)目報(bào)告 |
13-16周 |
多抗 13(Western檢測(cè)保證陽(yáng)性多抗制備) (Western guaranteed polyclonal antibodies) |
多肽設(shè)計(jì)與合成(多肽可到22aa, 75%) 多肽偶聯(lián) 動(dòng)物免疫及血清采集 ELISA檢測(cè) Western Blot檢測(cè) 抗原親和純化 |
peptide design and synthesis(up to 22aa,75%) peptide conjugation immunization and sera collection ELISA & Western Blot identification antigen affinity purification |
2-3mg多肽 2-5mg抗原親和純化抗體 Western Blot檢測(cè)結(jié)果 1-2ml免疫前血清 ELISA檢測(cè)結(jié)果 完整項(xiàng)目報(bào)告 |
15-18周 |
多抗 16 (Western和IHC檢測(cè)同時(shí)保證陽(yáng)性多抗制備) (Western and IHC guaranteed polyclonal antibodies) |
多肽設(shè)計(jì)與合成(多肽可到22aa, 75%) 多肽偶聯(lián) 動(dòng)物免疫及血清采集 ELISA檢測(cè) Western Blot和IHC檢測(cè) 抗原親和純化 |
peptide design and synthesis(up to 22aa,75%) peptide conjugation immunization and sera collection ELISA identification Western Blot & IHC identification antigen affinity purification |
2-3mg多肽 2-5mg抗原親和純化抗體 Western Blot和IHC檢測(cè)結(jié)果 1-2ml免疫前血清 ELISA檢測(cè)結(jié)果 完整項(xiàng)目報(bào)告 |
15-18周 |
單抗 1 (從蛋白抗原到腹水純化) (From protein to ascites purification) |
檢測(cè)蛋白的純度和濃度 動(dòng)物免疫及血清采集 ELISA檢測(cè) 細(xì)胞融合及 雜交瘤制備 亞型鑒定 腹水生產(chǎn)及純化 (2株雜交瘤) |
protein checked by SDS-PAGE immunization and sera collection ELISA identification fusion and hybridoma development isotype determination ascites production and purification (2 clones) |
50ul(1:10稀釋)免疫前血清 2-5株雜交瘤細(xì)胞 2-5mg純化抗體 (2株雜交瘤) ELISA檢測(cè)結(jié)果 亞型鑒定結(jié)果 完整項(xiàng)目報(bào)告 |
18-24周 |
單抗 2 (從多肽設(shè)計(jì)到腹水純化) (From peptide design to ascites purification) |
多肽設(shè)計(jì)與合成(12-22aa,85%) 多肽偶聯(lián) 動(dòng)物免疫及血清采集 ELISA檢測(cè) 細(xì)胞融合及 雜交瘤制備 亞型鑒定 腹水生產(chǎn)及純化 (2株雜交瘤) |
peptide design and synthesis(up to 22aa,75%) peptide conjugation immunization and sera collection ELISA identification fusion and hybridoma development isotype determination ascites production and purification (2 clones) |
2-3mg多肽 50ul(1:10稀釋)免疫前血清 2-5株雜交瘤細(xì)胞 2-5mg純化抗體 (2株雜交瘤) ELISA檢測(cè)結(jié)果 亞型鑒定結(jié)果 完整項(xiàng)目報(bào)告 |
20-26周 |
單抗 3 (從質(zhì)粒到腹水純化)(From plasmid to ascites purification) | 蛋白表達(dá)與純化 動(dòng)物免疫及血清采集 ELISA檢測(cè) 細(xì)胞融合及 雜交瘤制備 亞型鑒定 腹水生產(chǎn)及純化 (2株雜交瘤) |
protein expression and purification immunization and sera collection ELISA identification fusion and hybridoma development isotype determination ascites production and purification (2 clones) |
1mg表達(dá)蛋白 50ul(1:10稀釋)免疫前血清 2-5株雜交瘤細(xì)胞 2-5mg純化抗體 (2株雜交瘤) ELISA檢測(cè)結(jié)果 亞型鑒定結(jié)果 完整項(xiàng)目報(bào)告 |
20-26周 |
單抗 4 (從cDNA到腹水純化) (From cDNA to ascites purification) |
基因擴(kuò)增 載體構(gòu)建 蛋白表達(dá)與純化 動(dòng)物免疫及血清采集 ELISA檢測(cè) 細(xì)胞融合及 雜交瘤制備 亞型鑒定 腹水生產(chǎn)及純化 (2株雜交瘤) |
gene amplification vector construction protein expression and purification immunization and sera collection ELISA identification fusion and hybridoma development isotype determination ascites production and purification (2 clones) |
構(gòu)建的表達(dá)載體 1mg表達(dá)蛋白 50ul(1:10稀釋)免疫前血清 2-5株雜交瘤細(xì)胞 2-5mg純化抗體 (2株雜交瘤) ELISA檢測(cè)結(jié)果 亞型鑒定結(jié)果 完整項(xiàng)目報(bào)告 |
22-26周 |
單抗 5 (磷酸化單抗制備) (Phospho- specific monoclonal antibodies) |
多肽設(shè)計(jì)與合成(12-22aa,85%) 多肽偶聯(lián) 動(dòng)物免疫及血清采集 ELISA檢測(cè) 細(xì)胞融合及 雜交瘤制備 亞型鑒定 腹水生產(chǎn)及純化 (2株雜交瘤) |
peptide design and synthesis(up to 22aa,75%) peptide conjugation immunization and sera collection ELISA identification fusion and hybridoma development isotype determination ascites production and purification (2 clones) |
2-3mg多肽(非磷酸化和磷酸化) 50ul(1:10稀釋)免疫前血清 2-5株雜交瘤細(xì)胞 2-5mg純化抗體 (2株雜交瘤) ELISA檢測(cè)結(jié)果 亞型鑒定結(jié)果 完整項(xiàng)目報(bào)告 |
21-26周 |
單抗 6 (Western檢測(cè)保證陽(yáng)性單抗制備) (Western guaranteed monoclonal antibodies) |
多肽設(shè)計(jì)與合成(12-22aa,85%) 多肽偶聯(lián) 動(dòng)物免疫及血清采集 ELISA檢測(cè) 細(xì)胞融合及 雜交瘤制備 亞型鑒定 腹水生產(chǎn)及純化 (2株雜交瘤) |
peptide design and synthesis(up to 22aa,75%) peptide conjugation immunization and sera collection ELISA & Western Blot identification fusion and hybridoma development isotype determination ascites production and purification (2 clones) |
2-3mg多肽 Western Blot檢測(cè)結(jié)果 50ul(1:10稀釋)免疫前血清 2-5株雜交瘤細(xì)胞 2-5mg純化抗體 (2株雜交瘤) ELISA檢測(cè)結(jié)果 亞型鑒定結(jié)果 完整項(xiàng)目報(bào)告 |
20-26周 |