| 克隆類型 ： |
Mouse Monoclonal Antibody, PE-conjugated Antibody |
|
亞 型 ： |
mouse IgG2b |
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產品描述 ： |
The CD235ab (HIR2) antibody recognizes a N-terminal homologous portion of glycophorin-A (GPA) and glycophorin-B (GPB), which are single-pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities while GPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface acting as a barrier to cell fusion, therefore minimizing aggregation between red blood cells in the circulation. The CD235ab (HIR2) antibody agglutinates untreated RBC but failed to agglutinate papain-treated cells. This antibody primarly binds to GPA and weakly to GPB. CD235ab is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells. Mature erythrocytes are CD235ab positive whereas CD45 and CD71 negative. |
| 適用物種 ： |
H |
|
應用范圍 ： |
FC |
| 應用說明 ： |
FC with whole blood: 100 μl blood + 20 μl antibody; FC with cells: 10E6 cells + 20 μl antibody. FC: 1:200-1:1,000 |
| 別 名 ： |
CD235ab; CD235a; Glycophorin-A; PAS-2; Sialoglycoprotein alpha; MN sialoglycoprotein; GYPA; GPA; CD235B; Glycophorin-B; PAS-3; Sialoglycoprotein delta; SS-active sialoglycoprotein; GYPB; GPB |
|
形 態(tài) ： |
Each vial contains 2 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography. |
| 保存條件 ： |
Store at 4°C. Product is guaranteed 6 months from the date of shipment. |
|
產品文獻 ： |
Shen DC., et al. 1993. J. of Monoclonal Antibody. 9(2): 59; David M., et al. 2002. Leucocyte Typing VII: White Cell Differentiation Antigens. P577-582. |
| 產品鏈接 ： |
http://www.abbiotec.com/antibodies/cd235ab-hir2-antibody-0 |
| 標 記 物 ： |
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產品說明 ： |
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