| 克隆類型 ： |
Mouse Monoclonal Antibody, PE-conjugated Antibody |
|
亞 型 ： |
mouse IgG2a |
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產(chǎn)品描述 ： |
The CD3 (HIT3a) antibody recognizes the 17-19 kD ε-chain of CD3 within the CD3 antigen/T cell antigen receptor (TCR) complex. The CD3 antigen is expressed in the cell cytoplasm during the early stage of T cell development and is expressed on the cell membrane at the late stage. CD3 antigen is displayed on 60-80% of normal peripheral blood lymphocytes and 60-70% of thymocytes and plays an important role in signal transduction after antigen recognition by TCR. The clone HIT3a has a strong mitogenic effect at ng level on T lymphocyte proliferation in soluble or immobilized conditions and has an immunosuppressive effect at high dose. Conversely, the clone HIT3b has a strong mitogenic effect on T lymphocytes proliferation only under immobilized condition. NK cells also express the CD3 antigen in the cytoplasm, making the CD3 (HIT3a) antibody a helpful tool for analyzing the development relationship and the common precursor of both T cells and NK cells. |
| 適用物種 ： |
H |
|
應(yīng)用范圍 ： |
FC |
| 應(yīng)用說明 ： |
FC with whole blood: 100 μl blood + 20 μl antibody; FC with cells: 10E6 cells + 20 μl antibody. FC: 1:200-1:1,000 |
| 別 名 ： |
T-cell surface glycoprotein CD3; T-cell surface antigen T3/Leu-4; CD3; T3 |
|
形 態(tài) ： |
Each vial contains 2 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography. |
| 保存條件 ： |
Store at 4°C. Product is guaranteed 6 months from the date of shipment. |
|
產(chǎn)品文獻(xiàn) ： |
Shen DC. et al. 1993. ACTA Academia Medicinae Sinicae. 15(3):157; Schlossman S. et al., eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. P246, Oxford University Press, New York.; Ta |
| 產(chǎn)品鏈接 ： |
http://www.abbiotec.com/antibodies/cd3-hit3a-antibody-1 |
| 標(biāo) 記 物 ： |
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產(chǎn)品說明 ： |
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