產品貨號Reference:               CAN-PRE-4500
檢測方法Kit Type:                  Competitive ELISA
規(guī)格Kit Size:                          96-well break-apart microplate
靈敏度Sensitivity:                 0.05 ng/mL
檢測樣本Sample Type:         Human serum / 50 μL
檢測范圍Calibrator Range:  0.1–25.6 ng/mL
檢測時間Total Assay Time:  75 minutes

背景介紹：

孕烯醇酮(3β-Hydroxypregn-5-en-20-one)是甾體合成途徑中第一個由膽固醇衍生而來的甾體，是所有腎上腺和性腺類固醇的共同前體。它的產生是通過P-450SCC酶切割膽固醇C-20側鏈而在線粒體中產生的。孕烯醇酮一旦產生，可通過兩條甾體合成途徑加以利用。孕烯醇酮可以通過17-α-羥化酶的酶促作用轉化為17-OH孕烯醇酮，也可以通過3-β-羥基類固醇脫氫酶的酶促作用轉化為孕酮。
Pregnenolone (3β-hydroxypregn-5-en-20-one) is the first steroid to be derived from cholesterol in the pathway of steroidogenesis, and it is the common precursor for all of the adrenal and gonadal steroids. Its production occurs in the mitochondrion by cleavage of the C-20 side chain of cholesterol by the P-450SCC enzyme. Once produced, pregnenolone may be utilized by two pathways of steroidogenesis. Pregnenolone may either be converted to 17-OH pregnenolone via the enzymatic action of 17α-hydroxylase or to progesterone via the enzymatic action of 3β-hydroxysteroid dehydrogenase.